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- Title
Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey.
- Authors
BAYRAM, Davut; ARSLAN, Korhan; AKYÜZ, Bilal; İŞCAN, Kaan Muhsin
- Abstract
Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X²:40.75, p<0.001and X2:8.4, p<0.01).
- Subjects
TURKEY; TRANSCRIPTION factors; LEPTIN genetics; CATTLE diseases; GENETIC polymorphisms
- Publication
Veterinary Journal of Ankara University / Ankara Universitesi Veteriner Fakultesi Dergisi, 2017, Vol 64, Issue 4, p337
- ISSN
1300-0861
- Publication type
Article
- DOI
10.1501/vetfak_0000002818