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- Title
Polyamines produced by an extreme thermophile are essential for cell growth at high temperature.
- Authors
Sakamoto, Akihiko; Tamakoshi, Masatada; Moriya, Toshiyuki; Oshima, Tairo; Takao, Koichi; Sugita, Yoshiaki; Furuchi, Takemitsu; Niitsu, Masaru; Uemura, Takeshi; Igarashi, Kazuei; Kashiwagi, Keiko; Terui, Yusuke
- Abstract
An extreme thermophile, Thermus thermophilus grows at an optimum temperature of around 70°C and produces 16 different polyamines including long-chain and branched-chain polyamines. We found that the composition of polyamines in the thermophile cells changes with culture temperature. Long-chain and branched-chain polyamines (unusual polyamines) were increased in the cells grown at high temperature such as 80°C, but they were minor components in the cells grown at relatively lower temperature such as 60°C. The effects of polyamines on cell growth were studied using T. thermophilus HB8 Δ speA deficient in arginine decarboxylase. Cell growth of this mutant strain was significantly decreased at 70°C. This mutant strain cannot produce polyamines and grows poorly at 75°C. It was also determined whether polyamines are directly involved in protecting DNA from DNA double-strand breaks (DSBs) induced by heat. Polyamines protected DNA against double-strand breaks. Therefore, polyamines play essential roles in cell growth at extremely high temperature through maintaining a functional conformation of DNA against DSBs and depurination.
- Subjects
CELL growth; DOUBLE-strand DNA breaks; POLYAMINES; HIGH temperatures; THERMUS thermophilus
- Publication
Journal of Biochemistry, 2022, Vol 172, Issue 2, p109
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvac048