We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Mechanism of metribuzin tolerance: herbicide metabolism as a basis for tolerance in potatoes.
- Authors
Gawronski, S. W.; Haderlie, L. C.; Callihan, R. H.; Gawronska, Helena
- Abstract
14C-5(ring)-metribuzin [4-amino-6-<em>tert</em>-butyl-3(methylthio)-as triazin-5(4H)-one] metabolism was studied in 'Russet Burbank' and 'Chipbelle' potato (<em>Solanum tuberosum</em> L.) cultivars after root treatment in hydroponic solution. Plants were treated at the four- to five-leaf stage and harvested 1, 4, and 8 days later. Extraction was made by homogenizing dried samples in 80% methanol and partitioning against diethyl ether. Metabolites were partially characterized by thin-layer chromatography (t.l.c.) and compared to known standard chemicals, except for conjugates for which we had no synthetic standards. Ether-soluble chemicals [metribuzin and diketo (DK), deaminated (DA), and deaminated diketo (DADK) forms] comprised 55, 37, and 36% of the total 14C in Chipbelle plants and in Russet Burbank 30, 15, and 13% at 1, 4, and 8 days, respectively. Metribuzin and DK, DA, and DADK were present in all organs of each cultivar, but Russet Burbank leaves had only 5% as much free metribuzin as did Chipbelle. Most radioactivity in the leaves was a conjugate form which comprised 81 and 46% of the total 14C in Russet Burbank and Chipbelle leaf blades at 8 days, respectively. There were five water-soluble conjugates separated which included two ninhydrin-reacting compounds and three conjugates reacting as sugars. The major foliar conjugates reacted as sugars and the major root conjugates reacted as peptides or amino acid for both cultivars. Terminal (insoluble) residues comprised 63 and 66% of total radioactivity in roots of Russet Burbank and Chipbelle, respectively after 4 or 8 days. The major mechanism of Russet Burbank tolerance to metribuzin is conjugation of metribuzin or DA, DK, or DADK derivatives to plant sugars or peptides.
- Subjects
HERBICIDES; POTATOES; SOLANUM; METRIBUZIN; TRIAZINES; PEPTIDES
- Publication
Weed Research, 1986, Vol 26, Issue 5, p307
- ISSN
0043-1737
- Publication type
Article
- DOI
10.1111/j.1365-3180.1986.tb00712.x