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- Title
Mechanism of Calcium Ionophore and Phorbol Ester-Induced T-cell Activation.
- Authors
Hashimoto, S.; Takahashi, Y.; Tomita, Y.; Hayama, T.; Sawada, S.; Horie, T.; Mccombs, C. C.; Michalski, J. P.
- Abstract
We examined the role of monocytes in T-cell activation induced by phorbol myristate acetate (PMA) and calcium ionophore ionomycin. Depletion of monocytes from peripheral blood mononuclear cells (PBMC) was associated with the loss of interleukin-2 (IL-2) production, IL-2 receptor (IL-2R) expression and proliferation, in response to cither PMA or ionomycin. Addition of monocytes to highly purified T cells resulted in the complete reconstitution of IL-2 production, IL-2R expression and proliferation by PMA-stimulated lymphocytes. Exogenous IL-2, but not interleukin-1 (IL-1), could reconstitute the T-cell responsiveness. Addition of monocytes to highly purified T cells stimulated with ionomycin resulted in partial reconstitution of IL-2 production, IL-2R expression and proliferation. Similarly, the addition of exogenous IL-2 to ionomycin-stimulated T cells only partially reconstituted the response compared with PBMC. These results suggest that monocyte--T-cell interactions contribute to IL-2 production and IL-2R expression and are crucial events for PMA-induced T-cell proliferation. With ionomycin, monocytes play a role, in part, in inducing IL-2 production, IL-2R expression and proliferation. However, IL-2 is not a sufficient signal to induce T-cell proliferative response to ionomycin, suggesting that an IL-2-independent mechanism may exist in ionomyein-induced T-cell proliferation.
- Subjects
LEUCOCYTES; MONOCYTES; LYMPHOCYTES; INTERLEUKINS; CELL division; CELL proliferation
- Publication
Scandinavian Journal of Immunology, 1991, Vol 33, Issue 4, p393
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01787.x