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- Title
Goofy Coordinates the Acuity of Olfactory Signaling.
- Authors
Kaneko-Goto, Tomomi; Yuki Sato; Sayako Katada; Emi Kinameri; Sei-ichi Yoshihara; Atsushi Nishiyori; Kimura, Mitsuhiro; Fujita, Hiroko; Kazushige Touhara; Reed, Randall R.; Yoshihara, Yoshihiro
- Abstract
The basic scheme of odor perception and signaling from olfactory cilia to the brain is well understood. However, factors that affect olfactory acuity of an animal, the threshold sensitivity to odorants, are less well studied. Using signal sequence trap screening of a mouse olfactory epithelium cDNA library, we identified a novel molecule, Goofy, that is essential for olfactory acuity in mice. Goofy encodes an integral membrane protein with specific expression in the olfactory and vomeronasal sensory neurons and predominant localization to the Golgi compartment. Goo/y-deficient mice display aberrant olfactory phenotypes, including the impaired trafficking of adenylyl cyclase III, stunted olfactory cilia, and a higher threshold for physiological and behavioral responses to odorants. In addition, the expression of dominant-negative form of cAMP-dependent protein kinase results in shortening of olfactory cilia, implying a possible mechanistic link between cAMP and ciliogenesis in the olfactory sensory neurons. These results demonstrate that Goofy plays an important role in establishing the acuity of olfactory sensory signaling.
- Subjects
OLFACTORY bulb; SENSORY perception; CELLULAR signal transduction; ODORS; MEMBRANE proteins; ANTISENSE DNA; LABORATORY mice
- Publication
Journal of Neuroscience, 2013, Vol 33, Issue 32, p12987
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.4948-12.2013