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- Title
Dithiothreitol Separation of Newborn Rodent Dermis and Epidermis.
- Authors
Epstein Jr., Ervin H.; Munderloh, Neil H.; Fukuyama, Kimie
- Abstract
Incubation of newborn mouse skin in media containing 0.01M dithiothreitol at 4°C for 2 hr reduces dermal-epidermal cohesion, and the 2 layers may be separated readily. Unlike other methods of separation, this technique does not require heating, nonphysiologic pH or ionic strength, or addition of proteolytic enzymes. The spilt occurs beneath the basal lamina. The separated epidermal sheet retains the periodic acid Schiff-positive "basement membrane," and the basal cells can incorporate ³H-thymidine.
- Subjects
MICE; SKIN; RODENTS; EPIDERMIS; DERMIS; PROTEOLYTIC enzymes; BASAL lamina
- Publication
Journal of Investigative Dermatology, 1979, Vol 73, Issue 3, p207
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12513507