We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
BULLOUS PEMPHIGOID: ELUTION OF IN VIVO-FIXED ANTIBODY.
- Authors
Landry, Madeleine; Sams Jr., W. Mitchell; Jordon, Robert E.
- Abstract
Bullous pemphigoid is characterized by the presence of both circulating and tissue-fixed antibasement-membrane zone (BMZ) antibody. The present study determined the organ location and specificity of antibody fixed in vivo from a patient who died with active pemphigoid. Direct immunofluorescence (IF) of several organs revealed BMZ antibody only in the skin, esophagus, and vagina. BMZ antibody, however, was eluted with citric acid buffer (pH 2.2) from skin, esophagus, vagina, and trachea. The eluted antibody was similar to the circulating pemphigoid antibody in terms of IgG subclass, tubular pattern, and inability to fix complement. However, the two antibodies do not seem to share the same antigenic site or at least do not share equal affinity for the antigenic sites. Complement studies revealed activation of both the classic and the alternate pathways within the tissue and reduction of all complement components within the blister fluid.
- Subjects
IMMUNOGLOBULINS; IMMUNOFLUORESCENCE; CITRIC acid; SKIN; ESOPHAGUS; VAGINA; IMMUNOLOGY technique
- Publication
Journal of Investigative Dermatology, 1973, Vol 61, Issue 6, p348
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12676619