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- Title
ISOLATION AND PROPERTIES OF A GERMINATIVE AND A NON-GERMINATIVE CELL POPULATION FROM POSTEMBRYONIC MOUSE, RABBIT, AND HUMAN EPIDERMIS.
- Authors
Moore, J. T.; Karasek, M. A.
- Abstract
A method to separate two populations of cells (germinative and non-germinative) from the epidermis of the mouse, rabbit and human is presented. The procedure is both simple and physiological and is based upon the principle that one population of cells (germinative) will selectively attach to a collagen gel while the other (non-germinative) will remain in suspension. The positive identification of each cell population is made by measurement of the extent of incorporation of 3H- thymidine into DNA both <em>in vivo</em> and <em>in vitro</em>. Morphologic and metabolic studies confirm this identification. The chemical composition of each cell type has been determined. When compared to the non-germinative cells, the germinative population shows an increase in DNA, RNA, phosphate, and lipids, a decrease in carbohydrates, and an equal quantity of total protein. The germinative cells have three key enzymes in the pathway for DNA synthesis (thymidylate synthetase, thymidine kinase and cytidine diphospho-reductase); the non-germinative cells have no detectable levels of these enzymes.
- Subjects
CELL populations; EPIDERMIS; CYTOLOGY; MICE; RABBITS; EPITHELIUM
- Publication
Journal of Investigative Dermatology, 1971, Vol 56, Issue 4, p318
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12261079