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- Title
Effects of hepatitis B virus X protein on human T cell cytokines.
- Authors
Lou, XiaoLi; Hou, YanQiang; Liang, DongYu
- Abstract
Chronic infection with hepatitis B virus (HBV) plays a significant role in hepatocellular carcinoma development. To investigate the effect of hepatitis B virus X protein (HBx) on inflammatory cytokines of human T cell, a eukaryotic expression vector, HBx-pEGFP-C1, was constructed and transfected into the Jurkat human T-cell line. Jurkat cells were transfected transiently using Lipofectamine 2000 and activated by phytohemagglutinin (PHA). Interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), IL-4, IL-10, IL-13, and IL-14 mRNA was measured. The results showed that the vector HBx-pEGFP-C1 was successfully constructed, and HBx was expressed in Jurkat cells. Compared with a control group, mRNA of IL-1β and TNF-α was significantly elevated in the HBx-pEGFP-C1 group ( p < 0.05), while IL-4, IL-10, IL-13, and IL-14 mRNA was decreased ( p < 0.05). Therefore, transient overexpression of HBx promoted PHA-induced pro-inflammatory cytokine secretion and repressed anti-inflammatory cytokine secretion in human T cells.
- Subjects
HEPATITIS B virus; LIVER cancer; CYTOKINES; T cells; GENETIC vectors; PHYTOHEMAGGLUTININS
- Publication
Canadian Journal of Microbiology, 2013, Vol 59, Issue 9, p620
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2013-0259