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- Title
Characterization of 4 T1-like lytic bacteriophages that lyse Shiga-toxin Escherichia coli O157:H7.
- Authors
Niu, Yan D.; Stanford, Kim; Ackermann, Hans-W.; McAllister, Tim A.
- Abstract
Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC strains exhibiting extreme susceptibility (average multiplicity of infection (MOI) = 0.0003−0.0007). All phages had icosahedral heads with tapered, noncontractile tails, a morphology indicative of T1-like Siphoviridae. Genome size of all phages was ∼44 kb, but EcoRІ or HindIII digestion profiles differed among phages. Based on restriction enzyme digestion profiles, phages AHP24, AHS24, and AHP42 were more related (66.7%−82.4%) to each other than to AKS96, while AHP24 and AHS24, isolated from the same feedlot pen, exhibited the highest identity (88.9%−92.3%). Phages AHP24 and AHS24 exhibited the broadest host range and strongest lytic activity against STEC O157:H7, making them strong candidates for biocontrol of this bacterium in cattle.
- Subjects
BACTERIOPHAGES; BACTERIAL toxins; ESCHERICHIA coli O157:H7; MICROBIAL genetics; PHYSIOLOGICAL control systems; BACTERIAL diseases in animals; BACTERIAL genomes; BACTERIOPHAGE genomes
- Publication
Canadian Journal of Microbiology, 2012, Vol 58, Issue 7, p923
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w2012-063