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- Title
Synergistic antimicrobial activity of TZP4 with conventional antibiotics against antibiotic‐resistant Pseudomonas aeruginosa.
- Authors
Kim, Eun Young; Kumar, S. Dinesh; Ajish, Chelladurai; Lee, Chul Won; Shin, Sung‐Heui; Yang, Sungtae; Bang, Jeong Kyu; Shin, Song Yub
- Abstract
We recently synthesized a triazine‐derived amphipathic molecule, TZP4, which has potent antimicrobial activity against antibiotic‐resistant Pseudomonas aeruginosa (ARPA). Here, we investigated the synergistic antimicrobial effect of TZP4 in combination with four different conventional antibiotics each representing a distinct mechanism against ARPA. Checkerboard assay and time‐kill assays showed that the potent synergistic antimicrobial activity was observed only in TZP4/chloramphenicol and TZP4/ciprofloxacin combinations. Flow cytometry analysis for these combinations demonstrated that the synergistic antimicrobial activity was due to the increased permeability of bacterial cytoplasmic membrane. In addition, TZP4 was able to prevent and eradicate biofilms, which are one of the primary reasons for drug resistance caused by ARPA. Confocal laser scanning microscopy revealed that TZP4 significantly reduced mature ARPA biofilm biomass. Overall, our findings suggest that TZP4 is a potential adjuvant to antimicrobial chemotherapy and a potential antibiofilm agent to treat infections caused by ARPA.
- Subjects
UNITED States. Defense Advanced Research Projects Agency; PSEUDOMONAS aeruginosa; ANTI-infective agents; BACTERIAL cell walls; CARIOGENIC agents; PEPTIDE antibiotics; LASER microscopy; ADJUVANT chemotherapy; ANTIBIOTICS
- Publication
Bulletin of the Korean Chemical Society, 2022, Vol 43, Issue 5, p739
- ISSN
0253-2964
- Publication type
Article
- DOI
10.1002/bkcs.12517