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- Title
Color-Discriminating Retinal Configurations of Sensory Rhodopsin I by Photo-Irradiation Solid-State NMR Spectroscopy.
- Authors
Yomoda, Hiroki; Makino, Yoshiteru; Tomonaga, Yuya; Hidaka, Tetsurou; Kawamura, Izuru; Okitsu, Takashi; Wada, Akimori; Sudo, Yuki; Naito, Akira
- Abstract
SRI (sensory rhodopsin I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color-dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M-intermediate (attractant) transforms into a P-intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G-state. The photoreactions were monitored with the 13C NMR signals of [20-13C]retnal- SrSRI using in situ photo-irradiation solid-state NMR spectroscopy. The M-intermediate was trapped at −40 °C by illumination at 520 nm. It was transformed into the P-intermediate by subsequent illumination at 365 nm. These results reveal that the G-state could be directly transformed to the P-intermediate by illumination at 365 nm. Thus, the stationary trapped M- and P-intermediates are responsible for positive and negative phototaxis, respectively.
- Subjects
RHODOPSIN; PHOTOBIOLOGY; PHOTOCHEMISTRY; PHOTOTAXIS; CHEMICALS
- Publication
Angewandte Chemie, 2014, Vol 126, Issue 27, p7080
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201309258