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- Title
Combined Antibacterial Activity of Phage Lytic Proteins Holin and Lysin from Streptococcus suis Bacteriophage SMP.
- Authors
Shi, Yibo; Li, Ning; Yan, Yaxian; Wang, Hengan; Li, Yan; Lu, Chengping; Sun, Jianhe
- Abstract
Development of novel antibacterial agents is required to control infection with multidrug-resistant Streptococcus suis. HolSMP and LySMP, the holin and lysin of S. suis serotype 2 bacteriophage, named SMP, are responsible for lysis of host cells and release of progeny phage. HolSMP and LySMP expressed in Escherichia coli BL21(DE3) exerted efficient activity at 37 °C, pH 5.2, with addition of 0.8 % β-mercaptoethanol. Lytic spectra of purified HolSMP, LySMP or HolSMP + LySMP mixture were investigated. HolSMP, exhibiting a narrow lytic spectrum, was effective against Staphylococcus aureus and Bacillus subtilis, which were insensitive to LySMP. Moreover, HolSMP was identified as a promising antibacterial agent which was able to extend the spectrum of LySMP. The data suggest that combined use of holin and lysin could be a candidate strategy for resolution of drug resistance.
- Subjects
HOLINS; LYSINS; STREPTOCOCCUS suis; BACTERIOPHAGES; ESCHERICHIA coli
- Publication
Current Microbiology, 2012, Vol 65, Issue 1, p28
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-012-0119-2