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- Title
Heat-Resistance and Heat-Shock Response in the Nosocomial Pathogen Enterococcus faecium.
- Authors
Laport, Marinella Silva; da Silva, Marilene Ramos; Silva, Carla Costa; do Carmo de Freire Bastos, Maria; Giambiagi-deMarval, Marcia
- Abstract
We have characterized the heat-shock response of the nosocomial pathogen Enterococcus faecium. The growth of E. faecium cells was analyzed at different temperatures; little growth was observed at 50°C, and no growth at 52°C or 55°C. In agreement, a marked decrease of general protein synthesis was observed at 52°C, and very light synthesis was detected at 55°C. The heat resistance of E. faecium cells was analyzed by measuring the survival at temperatures higher than 52°C and, after 2 h of incubation, viable cells were still observed at 70°C. By Western blot analysis, two heat-induced proteins were identified as GroEL (65 kDa) and DnaK (75 kDa). Only one isoform for either GroEL or DnaK was found. The gene expression of these heat-shock proteins was also analyzed by pulsed-labeled experiments. The heat-induced proteins showed an increased rate of synthesis during the first 5 min, reaching the highest level of induction after 10 min and returning to the steady-state level after 20 min of heat treatment. Correspondence to: M. Giambiagi-deMarval; email: marciagm@micro.ufrj.br -->
- Subjects
HEAT shock proteins; ENTEROCOCCUS; CELLS; PROTEIN synthesis; GENE expression
- Publication
Current Microbiology, 2003, Vol 46, Issue 5, p0313
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-002-3828-0