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- Title
Homonuclear chemical shift correlation in rotating solids via RN<sup>?</sup><sub> n</sub> symmetry-based adiabatic RF pulse schemes.
- Authors
Riedel, Kerstin; Leppert, Jörg; Häfner, Sabine; Ohlenschläger, Oliver; Görlach, Matthias; Ramachandran, Ramadurai
- Abstract
The efficacy of RN? n symmetry-based adiabatic Zero-Quantum (ZQ) dipolar recoupling schemes for obtaining chemical shift correlation data at moderate magic angle spinning frequencies has been evaluated. RNsequences generally employ basic inversion elements that correspond to a net 180° rotation about the rotating framex-axis. It is shown here via numerical simulations and experimental measurements that it is also possible to achieve efficient ZQ dipolar recoupling via RNschemes employing adiabatic pulses. Such an approach was successfully used for obtaining13C chemical shift correlation spectra of a uniformly labelled sample of (CUG)97- a triplet repeat expansion RNA that has been implicated in the neuromuscular disease myotonic dystrophy. An analysis of the13C sugar carbon chemical shifts suggests, in agreement with our recent15N MAS-NMR studies, that this RNA adopts an A-helical conformation.
- Subjects
SPECTRUM analysis; NUCLEIC acids spectra; NEUROMUSCULAR diseases; NEUROLOGICAL disorders; DYSTROPHY; RNA
- Publication
Journal of Biomolecular NMR, 2004, Vol 30, Issue 4, p389
- ISSN
0925-2738
- Publication type
Article
- DOI
10.1007/s10858-004-4056-7