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- Title
Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11-3.
- Authors
Beg, Q K; Bhushan, B; Kapoor, M; Hoondal, G S
- Abstract
Streptomyces sp. QG-11-3, which produces a cellulase-free thermostable xylanase (96 IU ml-1) and a pectinase (46 IU ml-1), was isolated on Horikoshi medium supplemented with 1% w/v wheat bran. Carbon sources that favored xylanase production were rice bran (82 IU ml-1) and birch-wood xylan (81 IU ml-1); pectinase production was also stimulated by pectin and cotton seed cake (34 IU ml-1 each). The partially purified xylanase and pectinase were optimally active at 60°C. Both enzymes were 100% stable at 50°C for more than 24 h. The half-lives of xylanase and pectinase at 70, 75 and 80°C were 90, 75 and 9 min, and 90, 53 and 7 min, respectively. The optimum pH values for xylanase and pectinase were 8.6 and 3.0, respectively, at 60°C. Xylanase and pectinase were stable over a broad pH range between 5.4 and 9.4 and 2.0 to 9.0, respectively, retaining more than 85% of their activity. Ca2+ stimulated the activity of both enzymes up to 7%, whereas Cd2+, Co2+, Cr3+, iodoacetic acid and iodoacetamide inhibited xylanase up to 35% and pectinase up to 63%; at 1 mM, Hg2+ inhibited both enzymes completely. Journal of Industrial Microbiology & Biotechnology (2000) 24, 396–402.
- Subjects
XYLANASES; POLYGALACTURONASE; STREPTOMYCES
- Publication
Journal of Industrial Microbiology & Biotechnology, 2000, Vol 24, Issue 6, p396
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1038/sj.jim.7000010