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- Title
Conversion of indene to cis-(1S),(2R)-indandiol by mutants of Pseudomonas putida F1.
- Authors
Connors, N; Prevoznak, R; Chartrain, M; Reddy, J; Singhvi, R; Patel, Z; Olewinski, R; Salmon, P; Wilson, J; Greasham, R
- Abstract
Two mutation and selection methods were used to isolate mutants of Pseudomonas putida F1 which convert indene to cis-(1S),(2R)-indandiol in a toluene-independent fashion. Using soybean or silicone oil as a second phase to deliver indene to the culture, cis-(1S),(2R)-indandiol, cis-(1R),(2S)-indandiol, 1,2-indenediol (or the keto-hydroxy indan tautomer), and the monooxygenation products 1-indenol and 1-indanone were produced from indene as a function of time. Similarly the enantiomeric excess of the cis-(1S),(2R)-indandiol produced also increased with increasing time. In addition, mutants were isolated which produced cis-(1S),(2R)-indandiol of lower optical purity which corresponded to reduced levels of 1,2-indenediol. These data suggest this toluene dioxygenase produces cis-(1S),(2R)-indandiol of low optical purity and that cis-glycol dehydrogenase plays a role in resolving the two cis-1,2-indandiol enantiomers.
- Subjects
PSEUDOMONAS; INDENE; ENZYMES; OXYGENASES
- Publication
Journal of Industrial Microbiology & Biotechnology, 1997, Vol 18, Issue 6, p353
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1038/sj.jim.2900402