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- Title
野地瓜茎中3"种绿原酸提取工艺的 优化及抗氧化活性研究.
- Authors
刘永玲; 马风伟; 赵治兵; 杨怡; 方丽红; 谢国芳
- Abstract
The overall desired yield values of neochlorogenic acid,chlorogenic acid,and cryptochlorogenic acid were used as evaluation indexesto optimize their reflux extraction process using response surface methodology. The antioxidant activity of extracts from the stems of Ficus tikoua Bur. were evaluated by DPPH free radical and ABTS+ free radical scavenging. The results showed that the optimal conditions were as follows: 63% ethanol concentration,extraction temperature of 72 ℃, and a solid-liquid ratio of 1∶26 g/mL. The validation test showed that the OD value of the three components was 0.920 1±0.023 0,with a deviation of 0.85% from the predicted value(0.928 0). The half inhibitory concentrations(IC50)of DPPH and ABTS+ free radical scavenging activities were(24.32±0.52) μg/mL and(67.52±0.89) μg/mL,respectively. Relevance analysis showed that all three chlorogenic acid compoundscontributed significantly to DPPH and ABTS+ radical scavenging activities.
- Subjects
RESPONSE surfaces (Statistics); FREE radicals; CHLOROGENIC acid; DEBURRING; STATISTICAL correlation; ETHANOL
- Publication
Food Research & Development, 2021, Vol 42, Issue 18, p100
- ISSN
1005-6521
- Publication type
Article
- DOI
10.12161/j.issn.1005-6521.2021.18.015