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- Title
马源人乳头瘤病毒115型E6基因的遗传变异分析.
- Authors
帕丽旦·努尔兰; 佟盼盼; 宋小珍; 段汝丽; 邓海峰; 贾陈阳; 杨恩惠; 田澍瑶; 王一凡; 况 玲; 谢金鑫
- Abstract
Papillomavirus (PV) is an important pathogen infecting both human and animal, but the virus has strict host specificity. 14 species of papillomaviruses have been reported to infect horses to date. Of these, horse-origin PV, HPV-18, HPV-23 and EcPV-1 had been detected in horses of North Xinjiang, China. In order to understand the types of PV circulating in horses in North Xinjiang, 395 samples (205 nasal swabs, 140 fecal samples and 50 aborted fetal lung tissue) are collected from three large)scale breeding farms in Yili and Changji, and one equestrian club in Urumqi, and the viral metagenomic sequencing was applied to detect the types of PVs in horses. The resulting metagenomic data showed that HPV-115 was present in samples. According to the HPV-115 reference strain GC02, specific primers against E6 gene were designed to verify the results of viral metagenomics. PCR results showed that six nasal swabs of farm B in Yili were positive for HPV-115, with a positive rate was 7.3% (6/82), In addition, the positive rate of the virus in aborted fetal lung tissue of Yili horses collected from farm B was 46% (23/50), thereby indicating that HPV-115 might be a new pathogen causing of abortions in horse, Further investigation revealed that the horse-origin HPV-115 might spill over to workers in HPV-115-positive farm in Yili. Sequences analysis indicated that the homology of nucleotides and amino acid among our detected HPV-115 E6 was 99.0%-100%, 98.1%-100% respectively. It shared 98.4%-99.0% nucleotide sequence identity and 97.1%-99.0% amino acid sequence identity with E6 gene of human origin HPV-115 reference strain GC02, but shared 32.4%-43.8% nucleotide sequence identity and 34.6%-45.4% amino acid sequence identity with E6 gene of EcPVs. The phylo-genetic tree based on the sequences of E6 gene showed that 11 horse-origin and human-origin HPV-115 detected in this study were clustered in an independence lineage. These results provide new evidence that HPV-115 can infect horses, may act as a causative agent for abortions in Yili horses, and infect humans laboring in Yili mare stables.
- Subjects
XINJIANG Uygur Zizhiqu (China); URUMQI (China); AMINO acid sequence; NUCLEOTIDE sequence; FETAL tissues; PAPILLOMAVIRUSES; ABORTION; HUMAN origins; METAGENOMICS; HORSE breeding
- Publication
Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao, 2022, Vol 44, Issue 9, p1001
- ISSN
1008-0589
- Publication type
Article
- DOI
10.3969/j.issn.1008-0589.202112006