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- Title
High survival and hatching rates following vitrification of embryos at blastocyst stage: a bovine model study.
- Authors
Huang, Jack Y. J.; Jin-Tae Chung; Seang Lin Tan; Ri-Cheng Chian
- Abstract
Cryopreservation of embryos at the blastocyst stage may provide an effective method to increase the cumulative pregnancy rate for each treatment cycle of ovarian-stimulated IVF. The objective of this study was to evaluate the survival rate and hatching rate of bovine blastocysts following vitrification using a method designed for oocytes, with a view to introducing this methodology into human assisted reproduction technology and reproductive medicine. Bovine blastocysts were produced from abattoir materials subjected to in-vitro maturation and in-vitro fertilization. Survival rate of the bovine blastocysts was 100% (94/94) following vitrification using a method designed for oocyte cryopreservation. There was no difference in the hatching rate of the bovine blastocysts between control (62.5%: 60/96) and vitrified (61.7%: 58/94) groups, The number of dead cells in the blastocysts was not significantly different between control (5.0 ± 2.9) and vitrified (9.5 ± 4,0) groups. In conclusion, the results of this study indicate that bovine blastocysts can be vitrified successfully using a procedure designed for oocyte cryopreservation. It is possible that this method may also be successful for the cryopreservation of human embryos. A further study into this is currently being organized.
- Subjects
BLASTOCYST; CRYOPRESERVATION of organs, tissues, etc.; EMBRYOS; FERTILIZATION in vitro; OVARIES
- Publication
Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2007, Vol 14, Issue 4, p464
- ISSN
1472-6483
- Publication type
Article
- DOI
10.1016/S1472-6483(10)60894-2