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- Title
A Ratiometric Fluorescent Probe for pH Measurement over a Wide Range Composed of Three Types of Fluorophores Assembled on a DNA Scaffold.
- Authors
Nakata, Eiji; Gerelbaatar, Khongorzul; Asif, Mashal; Konishi, Hiroaki; Shibano, Yuya; Lin, Peng; Morii, Takashi
- Abstract
The desirable properties of the sophisticated fluorescent pH probe are ratiometric detection properties and a wide detection range. In this study, three types of fluorophores with different fluorescence properties were assembled on a DNA origami nanostructure. DNA nanostructure has the advantage of being a scaffold that can assemble different types of fluorophores with control over their number and position. The defined number of three different fluorophores, i.e., pH-sensitive fluorescein (CF) and Oregon Green (OG), and pH-insensitive tetramethylrhodamine (CR), assembled on the DNA scaffold provided a ratiometric fluorescent pH probe with a wide pH detection range that could cover the variation of intracellular pH.
- Subjects
FLUORESCENT probes; FLUOROPHORES; DNA folding; DNA; FLUORESCEIN; FLUORESCENCE
- Publication
Chemistry (2624-8549), 2023, Vol 5, Issue 3, p1832
- ISSN
2624-8549
- Publication type
Article
- DOI
10.3390/chemistry5030125