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- Title
Chamomile and Urtica dioica extracts improve immunological and histological alterations associated with polycystic ovarian syndrome in DHEA -induced mice.
- Authors
Shamsi, Maryam; Ganji, Ali; Mosayebi, Ghasem; Amirhoseiny, Ensieh Seif; Shohani, Sepideh; Ghazavi, Ali
- Abstract
Background: One of the novel mechanisms in the pathogenesis of Polycystic ovary syndrome (PCOS) is low-grade chronic inflammation. Chamomile (Matricaria recutita L.) and Nettle (Urtica dioica), with phytoestrogenic and antioxidant properties, are traditionally used to treat gynecological diseases. This study investigated the immune-modulating effects of these two plants. Methods: Following the induction of PCOS by subcutaneous injection (SC) of Dehydroepiandrosterone (DHEA) in BALB / C mice. Mice were treated in five groups: Sham, PCOS, PCOS + Chamomile, PCOS + Nettle, and PCOS + Chamomile and Nettle for 21 days. Ovarian morphology, blood antioxidant capacity, the abundance of Treg cells, and expression of matrix metalloproteinase-9 (MMP-9), transforming growth factor-ß (TGF-ß), cyclooxygenase-2 genes (COX-2), and tumor necrosis factor-alpha (TNF-α) were measured. Results: Folliculogenesis, Cystic follicles, and corpus luteum improved in the treatment groups (P < 0. 05). Treg cells in the DHEA group were significantly reduced compared to the Sham group (P < 0. 01). However, this decrease was not corrected in treatment groups (P > 0. 05). Total serum antioxidant capacity was significantly increased in the treatment group of Nettle and Chamomile + Nettle (P < 0. 05). The expression of MMP9 and TGFβ genes in the PCOS group was significantly higher than the Sham group (P < 0. 05), which the expression of MMP9 was corrected by treatment with Chamomile + Nettle extract (P < 0. 05). Conclusion: Chamomile and Nettle extract may be an effective supplement in improving the histological and immunological changes of PCOS. However, more research is needed to confirm its effectiveness in humans.
- Subjects
INFLAMMATION prevention; STINGING nettle; OVARIES; TRANSFORMING growth factors-beta; HUMAN reproduction; FASTING; STATISTICS; BIOMARKERS; ROMAN chamomile; POLYCYSTIC ovary syndrome; COMBINATION drug therapy; ANIMAL experimentation; ONE-way analysis of variance; ANTI-inflammatory agents; DEHYDROEPIANDROSTERONE; IMMUNOMODULATORS; IMMUNE system; ANTIOXIDANTS; REGULATORY T cells; BLOOD collection; BLOOD sugar; HYPOGLYCEMIC agents; GERMAN chamomile; MATRIX metalloproteinases; GENE expression; COMPARATIVE studies; RESEARCH funding; DESCRIPTIVE statistics; TUMOR necrosis factors; HISTOLOGICAL techniques; OVARIAN follicle; PLANT extracts; OXIDOREDUCTASES; DATA analysis software; DATA analysis; MICE; PHYTOESTROGENS; PHARMACODYNAMICS
- Publication
BMC Complementary Medicine & Therapies, 2023, Vol 23, Issue 1, p1
- ISSN
2662-7671
- Publication type
Article
- DOI
10.1186/s12906-023-03936-7