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- Title
Recovery of Senescent Endothelial Cells From Injury.
- Authors
Korybalska, Katarzyna; Kawka, Edyta; Kusch, Angelika; Aregger, Fabienne; Dragun, Duska; Jörres, Achim; Bręborowicz, Andrzej; Witowski, Janusz
- Abstract
Percutaneous coronary intervention is increasingly performed in elderly patients. Because the procedure is associated with endothelial cell (EC) denudation, we compared recovery of young and old ECs from scratch injuries inflicted in culture. Although senescent ECs displayed markedly reduced potential to proliferate and migrate, they repopulated the wounds as fast as young cells. Morphometric analysis revealed that senescent cells were significantly larger and as a result far fewer senescent cells managed to cover the lesion. Compared with young EC, senescent cells displayed increased expression of senescence-associated β-galactosidase, nitric oxide synthase (eNOS), and AKT kinase, and secreted increased amounts of growth factors (VEGF, TGF-β), cytokines (IL-6, IL-8, MCP-1), adhesion molecules (sICAM-1), and matrix proteins (fibronectin). This secretory phenotype rather than the rate of wound closure per se may contribute to unfavorable vascular remodeling in the elderly undergoing coronary catheterization.
- Subjects
ENDOTHELIAL cells; CELLULAR aging; PHYSIOLOGICAL aspects of aging; VASCULAR endothelial growth factors
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2013, Vol 68, Issue 3, p250
- ISSN
1079-5006
- Publication type
Article
- DOI
10.1093/gerona/gls169