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- Title
THE EFFECT OF CENTRIFUGATION ON URINARY PROTEIN ANALYSIS IN HEMATURIC AND NON‐HEMATURIC URINE SAMPLES.
- Authors
JES, Mubarek Mohamed; AYDIN, Almila ŞENAT; ABDİ, Eid Hassan; NEŞELİOĞLU, Salim; YILMAZ, Gülsen
- Abstract
Introduction: It is recommended that urine samples should be centrifuged prior to protein analysis. The present study was carried out to investigate the impact of centrifugation on protein levels by analysing urine samples with normal and elevated erythrocyte count. Material and Methods: 68 routine urine specimens were divided into 5 groups according to erythrocyte count (negative, +1, +2, +3, +4) based on urine strip analysis. Protein levels were measured in the same specimens before and after centrifugation at 1000 x g for 5 min. Total protein measurement was done by using Cobas 501 auto analyser. All statistical analyses were performed using IBM SPSS (version 22.0). Results: In our study, centrifuged urine had significantly lower levels of protein than uncentrifuged urine, regardless of erythrocyte count (P<0.001 all). The mean urinary protein levels of the groups before and after centrifugation were determined as 9.8mg/dL, 17.8 mg/dL, 18.6 mg/dLand 63.7 mg/dL and 8.7 mg/dL, 17.4 mg/dL, 16.5mg/dL and 61.9 mg/dl for negative, +1, +2, > +3 positive hematuric samples, respectively. Discussion: Protein levels in urine declined with centrifugation, regardless of hematuria levels determined, but the magnitude of the decline was largest when specimens had erythrocyte count over +3. Hemoglobine in heamaturic samples might cause false elevated protein levels and centrifugal processing causes these proteins to precipitate. Proteinuria and hematuria are findings in the urine that can be symptoms of kidney disease and we should be aware of the effect of centrifugation on urinary protein measurement.
- Subjects
URINALYSIS; PROTEIN analysis; CENTRIFUGATION
- Publication
Ankara Medical Journal, 2017, p18
- ISSN
2148-4570
- Publication type
Article