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- Title
超声协同复合酶法提取番茄红素及体外 模拟消化对抗氧化活性的影响.
- Authors
李昌宝; 辛明; 唐雅园; 孙健; 李丽; 零东宁; 何雪梅; 盛金凤; 刘国明; 邓军
- Abstract
【Objective】The present study was intent to optimize extraction process conditions of lycopene from cherry tomato and analyze its antioxidant activity,as well as provide a scientific foundation for further exploring cherry tomato lycopene.【Method】Based on single factor experiments,response surface methodology(RSM)was applied to optimize conditions of ultrasonic-assisted complex enzyme extraction of lycopene from cherry tomato. The changes in 1,1-diphenyl- 2-picrylhydrazyl(DPPH·)radical scavenging activity of lycopene after in vitro simulated digestion in artificial gastric juice and artificial intestinal juice were investigated as well.【Result】The rank of important influence factors on extraction rate of lycopene was ultrasonic time>enzymolysis temperature>composite enzyme amount>material to liquid ratio. The interaction between three factors(composite enzyme concentration,composite enzyme amount and ultrasonic time)and four interactions(material-to-liquid ratio and enzyme concentration,material-to-liquid ratio and ultrasonic time,composite enzyme amount and enzymatic hydrolysis temperature,and enzymatic hydrolysis temperature and ultrasonic time)had extremely significant influence on the lycopene extraction amount(P<0.01). The optimal extraction condition was as fol lows:material to liquid ratio of 1:40,composite enzyme amount of 3.6%,enzymolysis temperature of 54 °C and ultrasonic time of 22 min. Under this condition,the extraction quantity of cherry tomato lycopene was 410.94±1.78 μg/g,which was consistent with the predicted value of model(412.62 μg / g). The lycopene from cherry tomato showed dose-dependent scavenging activities against DPPH·,hydroxyl radical(·OH)and superoxide anions(O-2·)scavenging activity. The radical scavenging capacities of lycopene were greatly stronger than those of 2,6-di-tert-butyl-p-cresol(BHT)under the same concentration. The radical scavenging activity of lycopene to DPPH·was decreased after in vitro gastrointestinal digestion. When the concentration of lycopene was higher,the radical scavenging activity decrease was smaller.【Conclusion】 The ultrasonic-assisted complex enzyme extraction conditions of lycopene from cherry tomato is optimized by RSM. Lycopene from cherry tomatoes has strong antioxidant activity. Therefore,this research can provide a scientific foundation for deve-loping cherry tomato industry in future.
- Subjects
LYCOPENE; MULTIENZYME complexes; GASTRIC juice; TOMATOES; CHERRIES; DIGESTION
- Publication
Journal of Southern Agriculture, 2020, Vol 51, Issue 6, p1416
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j.issn.2095-1191.2020.06.023