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- Title
miR-218 promoted the apoptosis of human ovarian carcinoma cells via suppression of the WNT/β-catenin signaling pathway.
- Authors
Huang, Y.; Liang, S.-H.; Xiang, L.-B.; Han, X.-T.; Zhang, W.; Tang, J.; Wu, X.-H.; Zhang, M.-Q.
- Abstract
MicroRNA-218 (miR-218) is a short, noncoding RNA, with multiple biological functions. In this study, we aimed to investigate the potential effects of miR-218 on the apoptosis of human ovarian carcinoma cells and the underlying mechanisms by which miR-218 exerted its actions. After over-expressing miR-218 in human ovarian carcinoma (OVCAR3) cells, cell viability was determined by MTT method, cell apoptosis was observed by flow cytometry (FCM), mRNA expression of miR-218, Bcl2, Bax was measured by RT-PCR and protein expression levels of Wnt, tankyrase and β-catenin were quantified by Western blots. Over-expression of miR-218 potently suppressed cell viability and promoted the apoptosis of human ovarian carcinoma cells in a time-dependent manner. In addition, the down-regulation of tankyrase expression level was detected in miR-218-over-expressed cells. Following the block of the Wnt/β-catenin signaling pathway using the inhibitor XAV-939, the effects of miR-218 on the proliferation and apoptosis of human ovarian carcinoma cells were significantly suppressed. Augmenting expression of miR-218 and/or miRNA-218 mimicking therapeutics may provide viable avenue for the treatment of ovarian cancer.
- Subjects
OVARIAN cancer treatment; MICRORNA; APOPTOSIS; WNT proteins; CATENINS; GENE silencing
- Publication
Molecular Biology, 2017, Vol 51, Issue 4, p555
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1134/S0026893317030062