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- Title
In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate.
- Authors
Masashi Nagano; Sung-Sik Kang; Keisuke Koyama; Weiping Huang; Yojiro Yanagawa; Yoshiyuki Takahashi
- Abstract
As a preliminary study for the development of individual in vitro maturation (IVM) culture of bovine oocytes, a multi-well (MW) plate was used. Maturation, fertilization and development to blastocysts were examined and compared with those of IVM oocytes cultured in 50-µl droplets in groups and in 10-µl droplets individually. The maturation rates were similar in all experimental groups. Normal fertilization rates in MW and 50-µl droplets were similar, but lower in 10-µl droplets (p < 0.01). The blastocyst rate in 10-µl droplets tended to be lower than those in MW (p = 0.15) and 50-µl droplets (p = 0.19). These results indicate that an IVM system using MW supports the acquisition of developmental competence by bovine oocytes the same as conventional group IVM culture.
- Subjects
DEVELOPMENTAL biology; OVUM physiology; BLASTOCYST; FERTILIZATION in vitro; COMPARATIVE studies
- Publication
Japanese Journal of Veterinary Research, 2013, Vol 61, Issue 4, p149
- ISSN
0047-1917
- Publication type
Article