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- Title
Morphological, Biochemical, and Molecular Diversity Assessment of Egyptian Bottle Gourd Cultivars.
- Authors
Ibrahim, Ehab A.; Alhaithloul, Haifa A. S.; Shamseldin, Sahar A. M.; Awaly, Sara B. H.; El-Latif Hesham, Abd; Abdelkader, Mohamed F. M.; Alqahtani, Mesfer M.; Alzuaibr, Fahad Mohammed; Alasmari, Abdulrahman; Sukar, Noha A.; Diyasty, Mohamed Z.; Abdein, Mohamed A.
- Abstract
The genetic variability and relationships between ten bottle gourd cultivars were evaluated based on morphological, biochemical, and molecular parameters. The results displayed high variability among selected cultivars in terms of photosynthetic pigments, total free amino acids, total phenol content, isozymes pattern, and protein electrophoresis. Furthermore, di4erences in molecular markers were revealed by the SCoT technique. The peroxidase (POD) and polyphenyl oxidase (PPO) isozymes patterns did not detect signi<cant di4erences in bands among cultivars. The protein patterns revealed seventeen bands ranging from 126 to 9 kDa and <ve polymorphic bands representing 29.41%. On the other hand, eight SCoT primers were used to evaluate the genetic variability and relationships between the ten Egyptian bottle gourd cultivars. The results of SCoT analysis detected 44 amplicons with 50% polymorphism. In addition, the results of the phylogenetic tree that is constructed based on the similarity coe@cient revealed by SCoT analysis con<rm the results of biochemical analysis indicating a genetic relationship between the most e@cient bottle gourd cultivars (S1 and S2 cultivars). In addition, there is a genetic relationship among the less e@cient bottle gourd cultivars (S4 and S5 cultivars). These results could be bene<cial to distinguish among bottle gourd cultivars in the plant breeding programs.
- Subjects
LAGENARIA siceraria; CULTIVARS; GENETIC variation; PHOTOSYNTHETIC pigments; PLANT breeding
- Publication
Genetics Research, 2024, Vol 2024, p1
- ISSN
0016-6723
- Publication type
Article
- DOI
10.1155/2024/4182158