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- Title
BOB.1, CD79a and cyclin E are the most appropriate markers to discriminate classical Hodgkin’s lymphoma from primary mediastinal large B-cell lymphoma.
- Authors
Hoeller, Sylvia; Zihler, Deborah; Zlobec, Inti; Obermann, Ellen C.; Pileri, Stefano A.; Dirnhofer, Stephan; Tzankov, Alexandar
- Abstract
Hoeller S, Zihler D, Zlobec I, Obermann EC, Pileri SA, Dirnhofer S & Tzankov A (2010) Histopathology 56, 217–228 BOB.1, CD79a and cyclin E are the most appropriate markers to discriminate classical Hodgkin’s lymphoma from primary mediastinal large B-cell lymphoma Aims: To clarify which immunohistochemical markers could be helpful in distinguishing between classical Hodgkin’s lymphoma (cHL) and primary mediastinal B-cell lymphoma (PMBCL) to more narrowly define ‘B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and cHL’. Methods and results: Two hundred and 83 cHLs and 51 PMBCLs were analysed on validated tissue microarray platforms with antibodies to BOB.1, CD15, CD20, CD23, CD30, CD79a, cyclin E, LMP-1, MUM1p, p63 and Oct2. The marker cut-off scores were calculated using receiver–operating characteristic curves. Markers with the highest positive predictive value for cHL were: CD15, cyclin E, LMP-1 (all 100%), MUM1p (93%) and CD30 (83%). High sensitivity was achieved only by CD30 (92%) and cyclin E (79%). Nineteen percent of PMBCLs were also positive for CD30, which led to a lower specificity of CD30 as regards cHL (81%) compared with cyclin E (100%). The antibodies with the highest positive predictive value for PMBCL were: CD23 (98%), p63 (96%), BOB.1 (94%) and CD79a (90%), with high sensitivity for BOB.1 (100%), CD79a (89%) and p63 (82%). Conclusions: The use of at least three of the most accurate immunohistochemical markers, cyclin E, CD79a and BOB.1, may be helpful in the differential diagnosis of cHL and PMBCL.
- Subjects
LYMPHOMAS; B cell lymphoma; IMMUNOHISTOCHEMISTRY; PROTEIN microarrays; HISTOPATHOLOGY; DIFFERENTIAL diagnosis
- Publication
Histopathology, 2010, Vol 56, Issue 2, p217
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2009.03462.x