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- Title
Cytotoxic Effect of Aqueous and Alcoholic Extracts of Pterocarya fraxinifolia Leaves on K562 Cell Line.
- Authors
Abedigheshlaghi, Zahra; Monajemi, Ramesh; Yahyaabadi, Sima
- Abstract
Background: Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. Pterocarya fraxinifolia; (Juglandaceae) is widely distributed in northern area of Iran. The research evaluates the cytotoxic effect of n-butanol fraction, aqueous, methanolic and ethanolic extracts of P. fraxinifolia leaves on K562 cell line as a model of chronic myeloid leukemia. Materials and Methods: Leaves of P. fraxinifolia collected from Astara city and extraction using soxhlet method. K562 cells were cultured and treated with concentrations of extracts (12.5-400 µg/ml). Cytotoxicity of P. fraxinifolia extracts against K562 leukemia cells was estimated by the MTT test method. The absorbance was measured using an ELISA plate reader at 540 nm. Results: Ethanolic extract showed the highest cytotoxic effect (IC50=148.66±11.64 µg/ml) whereas n-butanol fraction extract were least cytotoxic effect (IC50=248.97±6.71 µg/ml) among the extracts. Aqueous and methanol extracts showed the cytotoxic effect with the IC50=183.14±4.71 and 226.02±6.08 µg/ml on K562 cell line. Both ethanolic and n-butanol fraction extracts exhibited a dose-dependent cytotoxic effect on K562 cell line. Conclusion: Considering the cytotoxic effects aqueous and alcoholic extracts of P. fraxinifolia leaves on K562 cells, the plant can be considered as a potential candidate for further studies on CML treatment6T
- Subjects
PLANT extracts; CYTOTOXINS; CELL lines; LEUKEMIA treatment; MYELOID leukemia; HEMATOPOIETIC stem cells; BLOOD platelets
- Publication
Zahedan Journal of Research in Medical Sciences, 2014, Vol 16, Issue 3, p1
- ISSN
2008-7977
- Publication type
Article