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- Title
Inflammatory cell profiles and T-lymphocyte subsets in chronic obstructive pulmonary disease and severe persistent asthma.
- Authors
Tsoumakidou, M.; Tzanakis, N.; Kyriakou, D.; Chrysofakis, G.; Siafakas, N. M.
- Abstract
Severe persistent asthma (SPA) and chronic obstructive pulmonary disease (COPD) are both associated with non-reversible airflow limitation and airway neutrophilia. To compare inflammatory cell profiles and T lymphocyte subsets between SPA and COPD patients with similar severity of airflow limitation. Sputum induction and lung function tests were performed in 15 COPD patients aged (mean±SD) 68±8 years, ex-smokers, mean forced expiratory volume in 1 s (FEV1) 45% of predicted (%pred) and 13 SPA aged 55±10 years, non-smokers, mean FEV1 49%pred. All patients were on inhaled steroid treatment. Eight asthmatics exhibited irreversible airflow limitation. Differential cell count, metachromatic cell count and double immunocytochemistry for the analysis of T lymphocyte subsets were performed on sputum slides. COPD patients had increased sputum neutrophils in comparison with SPA ( P<0.03), but similar to SPA with fixed obstruction. In COPD sputum neutrophils negatively correlated with the lung transfer factor for carbon monoxide ( KCO) ( r=−0.462, P=0.04). SPA showed significantly increased eosinophils and metachromatic cells vs. COPD patients ( P<0.04, P<0.007, respectively). Increased CD4/CD8 and decreased CD4-IFN-γ/CD4-IL4+ cell ratio ( P<0.001) were found in SPA vs. COPD. In SPA, CD4/CD8+ cell ratio correlated with sputum eosinophils ( r=0.567, P=0.04). In spite of treatment with inhaled steroids, SPA and COPD exhibit distinct sputum inflammatory cell patterns, although SPA with fixed airflow limitation and COPD patients have similar numbers of neutrophils.
- Subjects
OBSTRUCTIVE lung diseases; ASTHMA; LYMPHOCYTES; BRONCHIAL diseases; SPUTUM; PULMONARY function tests
- Publication
Clinical & Experimental Allergy, 2004, Vol 34, Issue 2, p234
- ISSN
0954-7894
- Publication type
Article
- DOI
10.1111/j.1365-2222.2004.01858.x