We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Simultaneous Determination of Four Ingredients in Guanxin Danshen Tablets by RP-HPLC.
- Authors
Yougen WU; Guihua ZENG; Daoan XIAO; Yujun WU; Yanjun CHEN; Juan LIU
- Abstract
[Objectives] This study aimed to establish a method for simultaneous determination of four ingredients in Guanxin Danshen tablets by RP-HPLC. [Methods] HPLC chromatography was adopted with column of Thermo Hypersil-Keystone CJS column (4.6 mm x 250 mm, 5 μm), mobile phase of acetonitrile (A) -0.03 mol/L ammonium acetate (pH adjusted to 2.4 with formic acid) (B), gradient elution (0 -5 min, 5% A; 5-10 min, 5% A→19% A; 10 -40 min, 19% A; 40 -68 min, 19% A→36% A; 68 -90 min, 36% A→95% A), flow rate of 1.0 mL/min and column temperature of 25 °C. [Results] The content of salvianic acid A sodium, protocatechuic aldehyde, salvianolic acid B and tanshinone IIA showed good linear relationship with chromatographic peak area in the range of 3. 310 -18. 66, 0. 039 50 - 0.237 0, 0.750 0 - 4.500, 0. 059 20 - 0. 355 0 peg, respectively. The recovery rate (n = 6) was 101.75%, 96. 86%, 104. 15% and 99.03%, respectively, and the USD was 1.52%, 2.81%, 1.80%, and 1.37% respectively. The established method has good precision, reproducibility and stability. [ Conclusions] This method can be used for the quality control of multiple ingredients of Guanxin Danshen tablets.
- Subjects
SALVIA miltiorrhiza; HIGH performance liquid chromatography; HYDROCHLOROTHIAZIDE; GRADIENT elution (Chromatography); FORMIC acid; QUALITY control
- Publication
Medicinal Plant, 2019, Vol 10, Issue 5, p18
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2019.05.005