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- Title
Molecular Characterization of the Nsp2 and ORF5s of PRRSV Strains in Sichuan China during 2012–2020.
- Authors
Zhao, Jun; Xu, Zhiwen; Xu, Tong; Zhou, Yuancheng; Li, Jiangling; Deng, Huidan; Li, Fengqing; Xu, Lei; Sun, Xiangang; Zhu, Ling
- Abstract
Simple Summary: PRRSV is one of the important pathogens that seriously affects the pig industry. Sichuan is a major pig breeding province in China and lacks long-term continuous surveillance of PRRSV infection in pigs. In this study, the prevalence of PRRSV in Sichuan was investigated based on a total of 539 samples of tissues, blood and aborted fetuses from 13 pig breeding areas in Sichuan. The genetic diversity of the virus currently circulating in Sichuan during 2012–2020 was studied by analyzing the genetic variation of Nsp2 and ORF5 genes. The detection results indicated that PRRSV was ubiquitous in the swine production in Sichuan, with 52.32% positive rate. Phylogenetic analysis of ORF5s showed that the PRRSV strains sequenced in this study belonged to PRRSV-1 and PRRSV-2 (lineage 1.5, 1.8, 3.5, 5.1 and 8.7). The data available in this study suggested that the genomes of prevalent PRRSV strains were complex and diverse. Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that poses a serious threat to the global pig industry. Sichuan Province is one of the largest pig breeding provinces in China. There is a lack of reports on the continuous surveillance and systematic analysis of prevalent strains of PRRSV in Sichuan Province in recent years. To fill this gap, a total of 539 samples were collected from 13 breeding regions in Sichuan during 2012–2020. The detection result showed that the positive rate of PRRSV was 52.32% (282/539). The ORF5s and Nsp2 were obtained and further analyzed, with Chinese reference strains downloaded from the GenBank. Phylogenetic analysis showed that the PRRSV strains sequenced in this study belonged to PRRSV-1 and PRRSV-2 (lineage 1, 3, 5 and 8). In total, 168 PRRSV-2 strains were selected for ORF5 analyses, and these strains were classified into sub-lineage 8.7 (HP-PRRSV), sub-lineage 5.1 (classical PRRSV), sub-lineage 1.8 (NADC30-like), sub-lineage 1.5 (NADC34-like) and sub-lineage 3.5 (QYYZ-like), accounting for 60.71% (102/168), 11.31% (19/168), 18.45% (31/168), 2.97% (5/168) and 6.55% (11/168) of the total analyzed strains, respectively. The Nsp2 of identified PRRSV strains exhibited a nucleotide identity of 44.5–100%, and an amino acid identity of 46.82–100%. The ORF5 of the identified PRRSV strains exhibited a nucleotide identity of 81.3–100%, and an amino acid identity of 78.5–100%. A sequence analysis of ORF5 revealed that the mutation sites of GP5 were mainly concentrated in HVR1 and HVR2 and the virulence sites. In summary, the HP-PRRSV, NADC30-like PRRSV, Classic-PRRSV, QYYZ-like PRRSV, NADC34-like PRRSV and PRRSV-1 strains exist simultaneously in pigs in Sichuan. NADC30-like PRRSV was gradually becoming the most prevalent genotype currently in Sichuan province. This study suggested that PRRSV strains in Sichuan were undergoing genomic divergence.
- Subjects
SICHUAN Sheng (China); CHINA; PORCINE reproductive &; respiratory syndrome; SWINE breeding; VIRUS diversity
- Publication
Animals (2076-2615), 2022, Vol 12, Issue 23, p3309
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani12233309