We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
IL-3 is an important differentiation factor for the development of prostaglandin E.
- Authors
Kuroda, Etsushi; Noguchi, Junko; Doi, Takahiro; Uematsu, Satoshi; Akira, Sizuo; Yamashita, Uki
- Abstract
We have previously reported that peritoneal and splenic macrophages from Th2-dominant BALB/c mice produced higher amounts of prostaglandin (PG) E than cells from C57BL/6 mice. In this study, we investigated how macrophages from BALB/c mice acquire the ability of enhanced PGE production, using bone marrow-derived macrophages differentiated by M-CSF, GM-CSF or IL-3. There is no strain difference in PGE production by GM-CSF- and M-CSF-differentiated macrophages; however, IL-3-differentiated macrophages from BALB/c mice produced higher amounts of PGE and lower amounts of type I cytokines than cells from C57BL/6 mice. IL-3-differentiated macrophages from BALB/c mice expressed larger amounts of mRNA of membrane-bound (microsomal) PGE synthase-1 (mPGES-1). The amounts of PGE produced by macrophages were significantly reduced in mPGES-1-deficient mice, and these mice displayed enhanced Th1 responses after Propionibacterium acnes treatment compared with wild-type mice. Microarray analysis revealed 63 genes that are differentially expressed more than fivefold in macrophages between C57BL/6 and BALB/c mice. These results indicate that mPGES-1-mediated PGE produced by macrophages regulates immune responses, and IL-3 is an important factor for the differentiation of macrophages that produce higher amounts of PGE through mPGES-1 activity in BALB/c mice.
- Publication
European Journal of Immunology, 2007, Vol 37, Issue 8, p2185
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200737041