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- Title
Production of gynogenetic diploid Polyodon spathula using fertilization with irradiated sperm followed by spontaneous diploidization and distant hybridization caused by heat shock.
- Authors
Zou, Y. C.; Wei, Q. W.; Zeng, L. B.; Li, P.
- Abstract
When paddlefish, Polyodon spathula, eggs were fertilized with UV irradiated (863 μW cm) sperm of Amur sturgeon, Acipenser schrenckii, viable gynogenetic diploid progeny with approximately 120 chromosomes occurred due to spontaneous diploidization of the maternal set of chromosomes (SDM). Distant hybridization of P. spathula eggs by A. schrenckii sperm induced inviable gynogenetic haploid progeny with approximately 60 chromosomes. When eggs gynogenetically activated by heterospecific sperm were heat-shocked with 37°C for 2 min at 18 min post-fertilization, a small proportion (7.8%) of viable gynogenetic diploid progeny appeared. The female inheritance of these gynogenetic diploid progeny was verified by chromosome analyses and microsatellite genotyping.
- Subjects
PADDLEFISH; FISH fertility; FERTILIZATION (Biology); SPECIES hybridization; FISH eggs; MICROSATELLITE repeats
- Publication
Journal of Applied Ichthyology, 2011, Vol 27, Issue 2, p505
- ISSN
0175-8659
- Publication type
Article
- DOI
10.1111/j.1439-0426.2011.01712.x