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- Title
Autologous blood transfusion augments impaired wound healing in diabetic mice by enhancing lncRNA H19 expression via the HIF-1α signaling pathway.
- Authors
Guo, Jian-Rong; Yin, Lei; Chen, Yong-Quan; Jin, Xiao-Ju; Zhou, Xun; Zhu, Na-Na; Liu, Xiao-Qian; Wei, Han-Wei; Duan, Li-Shuang
- Abstract
Background: Impaired wound healing frequently occurs in diabetes mellitus (DM) and is implicated in impaired angiogenesis. Long non-coding RNA (lncRNA) H19 has been reported as being reduced in DM and played a critical role in inducing angiogenesis. Thus, we hypothesized that H19 may affect impaired wound healing in streptozotocin (STZ)-induced diabetic mice transfused with autologous blood preserved in standard preservative fluid or modified preservative fluid. Methods: Fibroblasts in injured skin were isolated and cultured in vitro. After location of H19 in fibroblasts using fluorescence in situ hybridization (FISH), RNA-pull down, RNA immunoprecipitation (RIP), chromatin immunoprecipitation (ChIP), Co immunoprecipitation (COIP) and dual luciferase reporter gene assay were used to verify the binding of H19 to HIF-1α. Results: The modified preservative fluid preserved autologous blood increased the H19 expression in fibroblasts, and maintained better oxygen-carrying and oxygen release capacities as well as coagulation function. Furthermore, H19 promoted HIF-1α histone H3K4me3 methylation and increased HIF-1α expression by recruiting EZH2. H19 promoted fibroblast activation by activating HIF-1α signaling pathway in fibroblasts and enhanced wound healing in diabetic mice. Conclusions: Taken together, H19 accelerated fibroblast activation by recruiting EZH2-mediated histone methylation and modulating the HIF-1α signaling pathway, whereby augmenting the process of modified preservative fluid preserved autologous blood enhancing the postoperative wound healing in diabetic mice.
- Subjects
AUTOTRANSFUSION of blood; WOUND healing; FLUORESCENCE in situ hybridization; HISTONE methylation; NON-coding RNA; HISTONES; BLOOD coagulation factors
- Publication
Cell Communication & Signaling, 2018, Vol 16, Issue 1, pN.PAG
- ISSN
1478-811X
- Publication type
Article
- DOI
10.1186/s12964-018-0290-6