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- Title
Investigation of Neuroprotective Effect of n-Hexane Extract of Cissampelos pareira in Ischemic Stroke-Induced Nerve Damage and Underlying Mechanisms.
- Authors
Khalid, Muhammad Sohaib; Sarwar, Sadia; Shah, Fawad Ali; Alamro, Abir; Algamdi, Amani Ahmed; Alghamdi, Suad; Malik, Imran
- Abstract
Objective: Cissampelos pareira Linn. (Menispermaceae) has been reported for improving memory and cognitive behavior, and also as a folk remedy, for various neurodegenerative disorders. We investigated Cissampelos pareira for evaluating its neuroprotective effect on rodent model of focal cerebral ischemia. Methods: Male Sprague–Dawley rats (270-300 g) were subjected to permanent middle cerebral artery occlusion (MCAO). The animals were divided into five groups as control, MCAO, and MCAO + Cissampelos pareira (n -hexane, ethyl acetate (EtOAc), and methanol (MeOH) extracts; 50 mg/kg; n = 14). The expression of various inflammatory factors and enzymes including cyclooxygenase (COX-2), c-Jun N-terminal Kinase (p-JNK), and nuclear factor kappa-light-chain-enhancer of activated B cells (p-NF-κB) was detected in response to disease and extracts by immunohistochemistry and enzyme-linked immunosorbent assay. Results: The n -hexane extract (LD50 > 5.0 g/kg) reduced the infarction area significantly from in n -hexane extract + MCAO group, reversed neuronal death (P <.01), and relieved the neurobehavioral deficits. The reduced levels of antioxidant enzymes (GST, GSH, CAT, SOD, and GPx), in case of MCAO were significantly elevated in response to n-hexane extract. 1,2-benzenedicarboxylic acid was detected (through gas chromatography/mass spectrometry) as the major component in n -hexane extract. Conclusion: n -hexane extract has the potential to be of therapeutic value in stroke patients, and thus further studies are warranted to elucidate the neuroprotective effects of this extract.
- Subjects
HEXANE; ENZYME-linked immunosorbent assay; GENE enhancers; SPRAGUE Dawley rats; B cells; CEREBRAL ischemia; ETHYL acetate
- Publication
Natural Product Communications, 2024, Vol 19, Issue 4, p1
- ISSN
1934-578X
- Publication type
Article
- DOI
10.1177/1934578X241249069