We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle.
- Authors
Srinontong, Piyarat; Aengwanich, Worapol; Somphon, Sattabongkod; Khonwai, Siriyakorn; Nitsinsaku, Thanasorn; Wu, Zhiliang; Chalalai, Thanyakorn; Saraphol, Bhuripit; Srisanyong, Wilasinee
- Abstract
Background and Aims: Lipopolysaccharide (LPS) is a robust endotoxin known to activate the immune system in cattle. The objective of this study was to investigate the effect of LPS on the morphology, cell viability, malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC) of peripheral blood mononuclear cells (PBMCs) in Brahman and Brahman x Thai native crossbreed cattle. Materials and Methods: PBMCs were isolated from Brahman and Brahman x Thai native crossbreed cattle and treated with 0, 0.1, 1, and 10 µg/mL Escherichia coli LPS, respectively. Morphological changes in PBMCs were assessed at 24 and 48 h. In addition, we measured PBMC cell viability, MDA, NO, and TAC. Results: LPS stimulation caused cell deformation and partial PBMC area enlargement, but there were no differences between Brahman and Brahman x Thai native crossbreed cattle. Stimulation at all levels did not affect the viability of PBMCs (p > 0.05). MDA and NO levels were significantly higher in Brahman cattle than in Brahman Thai native crossbred cattle (p < 0.05). TAC was significantly higher in Brahman x Thai native crossbred cattle than in Brahman cattle (p < 0.05). Conclusion: Immune cells of crossbreed cattle have a higher activation response to LPS than those of purebred cattle, and native crossbreed beef cattle have a higher antioxidant capacity than purebred beef cattle. This result may explain why hybrid cattle of indigenous breeds are more resistant to disease than purebred cattle.
- Subjects
CATTLE crossbreeding; MONONUCLEAR leukocytes; ENDOTOXINS; ZEBUS; OXIDANT status; BEEF cattle; CATTLE breeds
- Publication
Veterinary World, 2024, Vol 17, Issue 4, p804
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2024.804-810