We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Developing immunological methods for detecting Macrobrachium rosenbergii nodavirus and extra small virus using a recombinant protein preparation.
- Authors
Wang, C‐S; Chang, C‐Y; Wen, C‐M
- Abstract
Macrobrachium rosenbergii nodavirus ( Mr NV) and extra small virus ( XSV) have been identified as the causative agents for white tail disease ( WTD) of M. rosenbergii. In this study, the gene sequences encoding Mr NV and XSV capsid proteins were separately ligated into the pGEX-4T-3 expression vector and transformed into Escherichia coli. After induction, glutathione-S-transferase ( GST)-tagged Mr NV and XSV fusion proteins were obtained with molecular masses of 68 and 43 kDa, respectively. Specific polyclonal antibodies for Mr NV and XSV against viral recombinant proteins and infected prawn tissues were verified using Western blotting. According to immunodot blot assay results, the detection sensitivities of antibodies were approximately 5 ng μL−1 for both recombinant proteins GST- Mr NV and GST- XSV. In additional, Mr NV and XSV were detected at dilution levels of 1:2560 and 1:640 in the infected prawn tissues, respectively. No cross-reactions with white spot syndrome virus or grouper nervous necrosis virus were observed using immunodot blot assays. Mr NV and XSV in infected muscle tissues were detected using immunohistochemistry. Although the detection limit of the immunodot blot assay was lower than that of nested reverse transcription polymerase chain reaction, these polyclonal antibodies can be useful for confirming Mr NV and XSV infections in field tests.
- Subjects
IMMUNODIAGNOSIS; IMMUNOHISTOCHEMISTRY; RECOMBINANT proteins; MACROBRACHIUM rosenbergii; NODAVIRUSES
- Publication
Journal of Fish Diseases, 2016, Vol 39, Issue 6, p715
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1111/jfd.12404