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- Title
CHARACTERIZATION AND ANTAGONISTIC POTENTIAL OF SOME NATIVE BIOFILM-FORMING AND SURFACTANT-PRODUCING BACILLUS SUBTILIS STRAINS AGAINST SIX PATHOTYPES OF RHIZOCTONIA SOLANI.
- Authors
Mousivand, M.; Jouzani, G. S.; Monazah, M.; Kowsari, M.
- Abstract
A screening strategy was developed to assess the potential of some Iranian native Bacillus sp. isolates collected from the rice rhizosphere and phyllosphere to control six pathotypes of the fungus Rhizoctonia solani using a hierarchical combination of different assays. Of 704 bacterial isolates tested, 240 (22% from the phyllosphere and 38% from the rhizosphere) showed more than 40% antifungal activity against the six R. solani pathotypes. The antagonistic feature among the isolates behaved as a continuous variable with normal distribution and antagonistic potential of the majority of the isolates tending to the mean (μ=49.58). These selected isolates showed significantly different mycelial growth inhibition. About 82% (197 isolates) of the selected bacterial strains produced biosurfactants, while only 18.2% (44 isolates) of them exhibited both biofilm formation and surfactant production, exhibing the highest antagonistic activities. Five of the most powerful antagonistic strains, in addition to biofilm and surfactants, produced different volatile and diffusible antifungal metabolites with different activities against the R. solani pathotypes, and were able to colonize the rice root surface at a level higher than the required threshold level. Biochemical and molecular (16SrDNA) characterization of three selected bacteria revealed them to be isolates of Bacillus subtilis. The difference in antagonistic mechanisms and optimal growth conditions of these three strains, suggests that their simultaneous application may lead to development of an effective biocontrol product.
- Subjects
RHIZOCTONIA diseases; RICE diseases &; pests treatment; BACILLUS subtilis; RHIZOCTONIA; RICE blast disease
- Publication
Journal of Plant Pathology, 2012, Vol 94, Issue 1, p171
- ISSN
1125-4653
- Publication type
Article