We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Acetylcholine Receptor Antibody in Myasthenia Gravis: Purification and Characterization.
- Authors
Lefvert, A.K.; Bergström, K.
- Abstract
Acetylcholine receptor antibody was measured in serum and IgG fractions from patients with myasthenia gravis using different analyses for antibody interfering with the toxin binding site and for antibody directed against other sites. No correlation was found between the concentration of receptor antibody as measured with the two assays. The different antibody activities also showed different isoelectric spectra. Receptor antibody was purified about 1000 times from IgG by affinity chromatography on a partially purified human skeletal muscle receptor preparation. The purified antibody was homogeneous in SDS-gel electrophoresis and showed a polyclonal pattern in agarose gel electrophoresis and isoelectric focusing. Both kinds of light chains were demonstrated. The results imply that the acetylcholine receptor antibody found in myasthenia gravis is heterogeneous and of multiclonal origin.
- Subjects
ACETYLCHOLINE; ELECTROPHORESIS; IMMUNOGLOBULIN G; MYASTHENIA gravis; IMMUNOGLOBULINS
- Publication
Scandinavian Journal of Immunology, 1978, Vol 8, Issue 6, p525
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1978.tb00552.x