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- Title
A simple method for the separation and detection of trace levels of buprofezin, flubendiamide and imidacloprid by NP-HPTLC and RP-HPTLC.
- Authors
Nareshkumar, B.; Sreeramulu, K.; Sangnalmath, Praveen U.; Gayatridevi, S.
- Abstract
A study was undertaken to evaluate the retention (RF and RM) and separation (ΔRF, RFα, α and RS) of buprofezin (B), flubendiamide (F) and imidacloprid (I) using n-hexane-acetone (6.5 : 3.5 v/v) in the case of NP-HPTLC and methanol-water (8 : 2 v/v) for RP-HPTLC as mobile phase. The study revealed that increasing the acetone content in NP-HPTLC and decreasing the water content in RP-HPTLC resulted in high resolution with increase in RF values for B, F and I. ΔRF > 0.04 and RS > 1.5 were achieved for all pairs of compounds (ΔRF(B-F) = 0.35, ΔRF(F-I) = 0.19, ΔRF(B-I) = 0.54, RS(B-F) = 4.12, RS(F-I) = 7.34, RS(B-I) = 2.02 using NP-HPTLC; ΔRF(F-B) = 0.23, ΔRF(I-F) = 0.26, ΔRF(I-B) = 0.49, RS(F-B) = 2.63, RS(I-F) = 2.97, RS(I-B) = 5.92 using RP-HPTLC). Imidacloprid was adsorbed strongly on NP-HPTLC layer and buprofezin on RP-HPTLC layer, as indicated by their high RM values. The maximum absorption of UV for B, F and I was found to be 252, 242 and 276 nm respectively. Stability analysis indicated that these compounds were stable up to 6 h in methanol and on the plates (NP-HPTLC and RP-HPTLC layers). This protocol is useful for toxicologists to detect a mixture of these insecticides in forensic as well as environmental samples.
- Subjects
BUPROFEZIN; IMIDACLOPRID; THIN layer chromatography; ACETONE; INSECTICIDES; ENVIRONMENTAL toxicology
- Publication
Current Science (00113891), 2018, Vol 115, Issue 5, p895
- ISSN
0011-3891
- Publication type
Article
- DOI
10.18520/cs/v115/i5/895-903