We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH.
- Authors
Vierstra, Jeff; Wang, Hao; John, Sam; Sandstrom, Richard; Stamatoyannopoulos, John A
- Abstract
It is currently not possible to resolve the genome-wide relationship of transcription factors (TFs) and nucleosomes at the level of individual chromatin templates despite rapidly increasing data on TF and nucleosome occupancy in the human genome. Here we describe DNase I-released fragment-length analysis of hypersensitivity (DNase-FLASH), an approach that directly couples mapping of TF occupancy, via quantification of DNA microfragments released from individual TF recognition sites in regulatory DNA, to the surrounding nucleosome architecture, via analysis of larger DNA fragments, in a single assay. DNase-FLASH enables coupling of individual TF footprints to nucleosome occupancy, identifying TFs that precisely demarcate the regulatory DNA-nucleosome interface.
- Subjects
TRANSCRIPTION factors; CHROMATIN; GENOMICS; DEOXYRIBONUCLEASES; GENETIC regulation; COUPLING reactions (Chemistry)
- Publication
Nature Methods, 2014, Vol 11, Issue 1, p66
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2713