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- Title
Asiatic acid attenuates malignancy of human metastatic ovarian cancer cells via inhibition of epithelial-to-mesenchymal transition.
- Authors
Xiufen Wang; Jie Sun; Feifei Liu; Yinghui Bian; Lili Miao; Xinbo Wang
- Abstract
Purpose: To investigate the anticancer effects of asiatic acid on human metastatic ovarian cancer cells. Methods: Human metastatic ovarian cancer cell line SKOV-3 was treated with various concentrations of asiatic acid for 24 and 48 h. Cell proliferation, migration, invasion and morphology were analyzed by CCK-8, Transwell and immunofluorescence assays, respectively. Epithelial-to-mesenchymal transitionrelated gene and protein expressions were analyzed by quantitative polymerase chain reaction (qPCR) and Western blotting. Results: Asiatic acid (10 µM) significantly suppressed SKOV-3 cell migration and invasion (both p < 0.01). Moreover, epithelial markers (E-cad and KRT-7/14/19) were elevated, while mesenchymal markers (vimetin, N-cad and ZEB1/2) were suppressed after asiatic acid treatment, at both mRNA and protein levels. Inhibition of epithelial-to-mesenchymal transition was further evidenced by immunofluorescence staining of pan-cytokeratin and F-actin. Conclusion: Asiatic acid attenuates the malignancy of human metastatic ovarian cancer cells via epithelial-to-mesenchymal transition inhibition, and thus, is a therapeutic agent for ovarian cancer management.
- Subjects
OVARIAN cancer treatment; AGLYCONE derivatives; POLYMERASE chain reaction; EPITHELIAL cells; MESENCHYMAL stem cells; MESSENGER RNA; CANCER cell migration; WESTERN immunoblotting
- Publication
Tropical Journal of Pharmaceutical Research, 2017, Vol 16, Issue 6, p1223
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v16i6.3