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- Title
Role of the NLRP3 inflammasome in the transient release of IL-1ß induced by monosodium urate crystals in human fibroblast-like synoviocytes.
- Authors
Shu-cong Zheng; Xiao-xia Zhu; Yu Xue; Li-hong Zhang; He-jian Zou; Jian-hua Qiu; Qiong Liu
- Abstract
Background: To investigate whether monosodium urate (MSU) crystals induce interleukin (IL)-1ß in human fibroblast-like synoviocytes (FLS), and whether the NLRP3 inflammasome is involved in the inflammatory mechanism. Methods: Human FLS isolated from explants of synovial tissue were stimulated with MSU crystals (0.001 to 0.5 mg/ml) for different time course (6 hours to 48 hours). The expressions of IL-1ß, IL-6, TNF-α and NLRP3 were evaluated with ELISA, Western blot and quantitative real-time PCR. Results: Exposure of FLS to MSU crystals transiently induced a significant increase in IL-1ß expression in culture medium with a peak at 6 h. The mRNA level of IL-1ß in the FLS cells had a similar pattern at this time point. Changes in IL-6 and TNF-α expression were not observed. Simultaneously, intercellular pro-IL-1ß was detected at 6 h. Furthermore, MSU crystals also induced NLRP3 mRNA and protein expression at 6 h to 48 h after MSU treatment. Conclusions: MSU crystals directly increased IL-1ß and intercellular NLRP3 expression in FLS cells. It is suggested that the NLRP3 inflammasome may be associated with IL-1ß in FLS treated with MSU. Altogether, MSU could induce production and release of IL-1ß through the NLRP3 inflammasome in human synoviocytes.
- Subjects
INTERLEUKIN-1; ACUTE phase proteins; INTERLEUKINS; SODIUM compounds; ALKALI metal compounds
- Publication
Journal of Inflammation, 2015, Vol 12, Issue 1, p1
- ISSN
1476-9255
- Publication type
Article
- DOI
10.1186/s12950-015-0070-7