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- Title
Effects of Mixed Culture of Human Isolates on Grade of Their Growth and Their Response to Honey.
- Authors
Al-Waili, Noori S.; Akmal, Mohammod; Al-Waili, Faiza S.; Ali, Amjed; Saloom, Khelod Y.; Al Waili, Thia N.; Al-Waili, Ali N.; Al-Waili, Hamza
- Abstract
BACKGROUND The present study investigated activity of honey towards high dose of single and mixed microbial culture when isolates were grown in media containing honey. The study showed effect of mixed microbial culture on their growth and their response to honey. MATERIAL AND METHODS 1) 10 ul specimens of Stapylococcus aureus (S. aureus), Streptococcus pyogens (S. pyogenes), E.coli and Candida albicans (C. albicans) were cultured into 10 ml broth containing 10-100% (w/v) honey concentrations. 2) Six types of mixed microbial cultures were prepared by culturing the isolates with each other onto broth (control) and broth containing various concentrations of honey (10-100% w/v). Microbial growth was assessed on solid plate media after 24 hours' incubation. RESULTS Honey (30-70%) prevented growth of 10 ul specimens of all the isolates. Greater reduction in growth of E.coli was observed when cultured with S. aureus. Culturing of S. aureus with S. pyogens, C. albicans, or E.coli increased its sensitivity to honey. S. aureus and S. pyogenes increased the sensitivity of C. albicans to honey while E. coli and C. albicans deceased the sensitivity of S. pyogenes. CONCLUSION Honey prevents and inhibits growth of single and mixed pathogenic isolates. Mixed microbial culture affects growth of the isolates and increases their sensitivity to honey.
- Subjects
HONEY; MIXED culture (Microbiology); STAPHYLOCOCCUS aureus; STREPTOCOCCUS pyogenes; ESCHERICHIA coli; CANDIDA albicans
- Publication
Malaysian Journal of Medical Sciences, 2007, Vol 14, Issue 1, p119
- ISSN
1394-195X
- Publication type
Article