The article focuses on the genome of Naegleria gruberi (N. gruberi), a free-living protist in the class Heterolobosea. It mentions that proteins in the nuclear genome were compared with 17 proteomes. It states that 40% of the 4,133 proteins belong to eukaryotes and most are hypothetical proteins. It says that the analysis of the N. gruberi genome is a significant comparative model for Naegleria fowleri (N. fowleri).