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- Title
Synthesis of bifunctional TiO@SiO-B(OH)@FeO@TiO sandwich-like nanosheets for sequential selective enrichment of phosphopeptides and glycopeptides for mass spectrometric analysis.
- Authors
Xu, Dongpo; Gao, Mingxia; Deng, Chunhui; Zhang, Xiangmin
- Abstract
In this work, the bifunctional TiO@SiO-B(OH)@FeO@TiO sandwich-like nanosheets were designed and synthesized for the sequential selective enrichment of phosphopeptides and glycopeptides. Due to the bifunctional property of the titanium dioxide and the boronic acid group, the nanosheets were successfully applied to the enrichment of phosphopeptides and glycopeptides sequentially, evaluated by capturing phosphopeptides from tryptic digestion of model phosphoprotein bovine β-casein diluted to 0.02 ng/μL (8 × 10 mol/μL) and glycopeptides from tryptic digestion of model glycoprotein horseradish peroxidase (HRP) diluted to 0.1 ng/μL (2.5 × 10 mol/μL). The enrichment selectivity of the bifunctional nanosheets was evaluated by capturing phosphopeptides from a peptide mixture of β-casein and bovine serum albumin (BSA) with the molar ratio of 1:1000 (8.3 × 10 mol of β-casein and 8.3 × 10 mol of BSA in 100 μL) and glycopeptides from a peptide mixture of HRP and BSA up to the ratio of 1:50 (5.0 × 10 mol of HRP and 2.5 × 10 mol of BSA in 100 μL). [Figure not available: see fulltext.]
- Subjects
CHEMICAL synthesis; PHOSPHOPEPTIDES; GLYCOPEPTIDES; MASS spectrometry; GLYCOPROTEINS; PHOSPHOPROTEINS; PHOSPHOPROTEIN phosphatases
- Publication
Analytical & Bioanalytical Chemistry, 2016, Vol 408, Issue 20, p5489
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-016-9647-0