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- Title
Stable recombinant alpaca antibodies for detection of Tulip virus X.
- Authors
Jules Beekwilder; Adèle van Houwelingen; Arjen Speksnijder
- Abstract
Abstract For detection of the plant pathogenic Tulip virus X (TuVX), a panel of six recombinant antibodies was identified. To this end, a repertoire of variable domains from heavy-chain immunoglobulins (VHH) was cloned from an alpaca, which had been immunized with TuVX. Binding domains were selected by phage display and panning on immobilized TuVX particles. Recombinant VHH antibodies were tested for sensitivity in a sandwich ELISA, and were demonstrated to be readily able to distinguish TuVX-infected tulip leaf material from uninfected leafs. No cross-reactivity of the VHH antibodies to related flexiviridae was observed. Recombinant VHHs maintained their reactivity upon storage at −20°C for over a year. The effect of incubation at higher temperatures for prolonged time was studied. Two out of three VHH proteins retained activity after several weeks of storage at 37°C.
- Subjects
PHYTOPATHOGENIC microorganisms; TULIP diseases &; pests; IMMUNOGLOBULINS; ALPACA
- Publication
European Journal of Plant Pathology, 2008, Vol 121, Issue 4, p477
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-007-9265-y