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- Title
Determining resistance to Pseudomonas syringae in tomato, a comparison of different molecular markers.
- Authors
Orsi, Ilaria; Malatrasi, Marina; Belfanti, Enrico; Gullì, Mariolina; Marmiroli, Nelson
- Abstract
Pseudomonas syringae pv. tomato ( Pst) is the causal agent of bacterial speck disease in tomato. Resistance to Pst is determined by Pto, a single resistance gene that belongs to a multi-gene family clustered on chromosome 5. Pst-resistant phenotypes in cultivated tomato are determined by a semi-dominant allele of Solanum pimpinellifolium, which was introgressed into Solanum lycopersicum in the past century. Seed companies, which are continuously interested in producing resistant varieties, can benefit from genetic markers closely linked to the Pto locus in breeding programs based on marker-assisted selection. In this research, three sequence characterised amplified region markers have been developed for identification of resistant and susceptible genotypes of Solanum lycopersicum. A cleaved amplified polymorphic sequence marker has been adapted to a real-time polymerase chain reaction platform using high-resolution melting curve analysis. Application to a tomato population for breeding programs is described. Advantages and disadvantages of the different markers are discussed.
- Subjects
PSEUDOMONAS syringae; TOMATO disease &; pest resistance; BACTERIAL diseases of plants; TOMATO genetics; PLANT chromosomes; TOMATO varieties; GENETIC markers in plants; SELECTION (Plant breeding)
- Publication
Molecular Breeding, 2012, Vol 30, Issue 2, p967
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-011-9681-8